Antioxidant Activity Test of The Aqueous Fraction of Tetanus Leaves (Leea aequata L.) Using The DPPH Method
Main Article Content
Abstract
Antioxidants play a crucial role in neutralizing free radicals, thereby helping to prevent cell damage and maintain overall health. This study aims to evaluate the antioxidant activity of the aqueous fraction of Tetanus (Leea aequata L.) leaf extract using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. The extraction process was carried out through maceration using ethanol as a solvent, followed by aqueous fractionation. Antioxidant activity was measured based on the extract’s ability to scavenge DPPH free radicals, expressed as the IC₅₀ value. The results showed that the aqueous fraction of Tetanus leaf extract exhibited high antioxidant activity, with an IC₅₀ value of 30.23 mg/L. The total phenolic and flavonoid contents in the aqueous fraction were 460.70 mg GAE/100g and 12,621.14 mg QE/100g, respectively. These findings align with pre vious studies indicating that the ethanolic extract of Leea angulata leaves also possesses strong antioxidant activity with a similar IC₅₀ value. Therefore, the aqueous fraction of Tetanus leaf extract has the potential to be an effective natural antioxidant source and can be further developed as a raw material for health supplements or pharmaceutical products.
Article Details
References
Anggarani, A. M., Ilmiah, M. & Nasyaya Mahfudhah, D. Antioxidant Activity of Several Types of Onions and Its Potensial as Health Supplements. Indones. J. Chem. Sci. 12, 103–111 (2023).
Pharmacia, J., Waluya, M., Amaliah, R., Pusmarani, J. & Nasir, N. H. Skrining Fitokimia dan Uji Aktivitas Antioksidan Fraksi Air , Etil Asetat dan n - Heksan Kulit Batang Kayu Jawa ( Lannea grandis ( Dennst .) Engl ) dengan Metode FRAP ( Ferric Reducing Antioxidant Power ) Phytochemical Screening and Antioxidant Activity A. 3, (2024).
Retnaningtyas, Y., Wulandari, L. & Fauzan Arrozi. AKTIVITAS ANTIOKSIDAN FRAKSI AIR DAN FRAKSI ETER KOMBINASI EKSTRAK METANOL DAUN KOPI ARABIKA (Coffea arabica L.) DAN KULIT BATANG KAYU MANIS (Cinnamomum burmannii Nees ex BI.). J. Tumbuh. Obat Indones. 16, 11–18 (2023).
Pusmarani, J., Ulfa, U., Dewi, C. & Nasir, N. H. Aktivitas Antioksidan Fraksi Air, Etil Asetat dan N-Heksan Kulit Pisang Raja (Musa paradisiaca var. Sapientum). J. Mandala Pharmacon Indones. 8, 275–283 (2022).
Sari, W. A. et al. UJI AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL AKAR BAJAKAH TAMPALA ( Spatholobus littoralis Hassk ) MENGGUNAKAN. 2, 374–384 (2024).
Wijaya, A., Rifki Widiastuti, N., Novi Rahmadani, A. & studi Diploma III Farmasi Akademi Farmasi Indonesia Yogyakarta, P. Antioxidant Activity of Water, Ethyl Acetate And Chloroform Fraction of Papaya (Carica papaya L.) Leaf Extract By DPPH Method. J. Jamu Kusuma 3, 2798–0332 (2023).
Misfadhila, S., Azizah, Z. & Maisarah, L. Penggunaan Metode DPPH dalam Penentuan Aktivitas Antioksidan Ekstrak Metanol dan Fraksi Daun Sukun (Artocarpus Altilis (Parkinson Ex F. A. Zorn) Fosberg). J. Farm. Higea 11, 75–82 (2019).
Harahap, U. et al. Antioxidant and antibacterial activities of ethanol extract of Vernonia amygdalina Delile. Leaves. in AIP Conference Proceedings vol. 2342 080011 (AIP Publishing LLC, 2021).
Dalimunthe, A. et al. The effect of extraction methods towards antioxidant activity of ethanol extract of Picria fel-terrae Lour. Herbs. in IOP Conference Series: Earth and Environmental Science vol. 1115 012040 (IOP Publishing, 2022).
Vrca, I. et al. Identification of Phenolic Compounds Present in Three Speedwell (Veronica L.) Species and Their Antioxidant Potential. Antioxidants 13, 1–15 (2024).
Susilowati & Wulandari, S. Aktivitas Antioksidan Fraksi Etil Asetat dan Fraksi Air Ekstrak Etanol Daun Salam ( Syzygium polyanthum ( Wight .) Walp .) dengan Metode DPPH ( 1 , 1 Difenil-2 pikrilhidrazil. Indones. J. Med. Sci. 6, 39–44 (2019).