Evaluation of Anti-Inflammatory Activities of Ethanolic Extract of Curcuma longa Rhizome in Rodent Models
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Abstract
Inflammation is a biological response to tissue injury or infection that often requires long-term management. Although synthetic anti-inflammatory drugs are effective, their prolonged use may cause adverse effects, highlighting the need for safer natural alternatives. Curcuma longa L. has been traditionally used for the treatment of inflammatory conditions. This study aimed to evaluate the anti-inflammatory activity of the ethanolic extract of Curcuma longa rhizome using a carrageenan-induced paw edema model in rats. An in vivo experimental study was conducted using male white rats (Rattus norvegicus). The animals were divided into five groups: negative control (0.5% CMC), positive control (diclofenac sodium 10 mg/kg BW), and three treatment groups receiving the ethanolic extract at doses of 100, 200, and 400 mg/kg BW. Paw edema was induced by carrageenan injection, and paw diameter was measured at 0, 2, 4, and 6 hours. Anti-inflammatory activity was expressed as the percentage of inflammation. The ethanolic extract of Curcuma longa significantly reduced inflammation compared to the negative control at all observation times. The 200 mg/kg BW dose exhibited the most effective and consistent anti-inflammatory activity, while a higher dose did not provide additional benefit. The ethanolic extract of Curcuma longa rhizome possesses significant anti-inflammatory activity, with an optimal effect observed at 200 mg/kg BW, supporting its potential as a natural anti-inflammatory agent.
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References
Begum, M. R. & Hossain, M. A. Validity and reliability of visual analogue scale (VAS) for pain measurement. J. Med. Case Rep. Rev. 2, (2019).
Lin, C.-Y. et al. Pre-existing humoral immunity to human common cold coronaviruses negatively impacts the protective SARS-CoV-2 antibody response. Cell Host Microbe 30, 83–96.e4 (2022).
Fratiwi, N., Saranani, S., Agastia, G. & Isrul, M. Aktivitas antiinflamasi ekstrak etanol daun kirinyuh (Chromolaena odorata L.) dan pengaruhnya terhadap kadar interleukin 6 (IL-6) pada tikus jantan galur wistar. J. Pharm. Mandala Waluya 1, 54–67 (2022).
Al-Qahtani, A. A., Alhamlan, F. S. & Al-Qahtani, A. A. Pro-inflammatory and anti-inflammatory interleukins in infectious diseases: A comprehensive review. Trop. Med. Infect. Dis. 9, 13 (2024).
Lubis, M. F. et al. Acute toxicity and antifungal activity of the ointment Murraya koenigii ethanol extract. Rasayan J. Chem. 15, 256–261 (2022).
Alam, B. et al. Antioxidant, analgesic and anti-inflammatory activities of the methanolic extract of Piper betle leaves. Avicenna J. Phytomedicine 3, 112–125 (2013).
Khan, B. A. et al. Fabrication, physical characterizations, and in vitro, in vivo evaluation of ginger extract-loaded gelatin/poly (vinyl alcohol) hydrogel films against burn wound healing in animal model. AAPS PharmSciTech 21, 1–10 (2020).
Kaban, V. E. Uji Formula Krim Ekstrak VCO Kunyit dari Rimpang Kunyit (Curcuma domestica Val.) Terhadap Penghambatan Perkembangan Sel Melanoma. (Universitas Sumatera Utara, 2021).
Kaban, V. E. et al. The effect of turmeric parent extract gel (Curcuma longa Linn) on incision wound healing in male white rats (Rattus norvegicus). J. Pharm. Sci. 616–627 (2024).
He, X. et al. Curcumin-loaded mesenchymal stem cell–derived exosomes efficiently attenuate proliferation and inflammatory response in rheumatoid arthritis fibroblast-like synoviocytes. Appl. Biochem. Biotechnol. 195, 51–67 (2023).
Kusumaningrum, H. P., Kusdiyantini, E. & Pujiyanto, S. Kualitas simplisia tanaman biofarmaka Curcuma domestica setelah proses pemanasan pada suhu dan waktu bervariasi. Bioma Berk. Ilm. Biol. 17, 27–33 (2015).
Panjaitan, J., Girsang, E. & Chiuman, L. Effectiveness of temulawak (Curcuma xanthorrhiza) ointments as wound healing agents in Wistar rats. J. Teknol. Lab. 11, 95–104 (2022).
da Silva, A. C. et al. Impact of curcumin nanoformulation on its antimicrobial activity. Trends Food Sci. Technol. 72, 74–82 (2018).
Kaban, V. E., Aritonang, J. O., Hasibuan, Y. C. & Meliala, D. I. P. Efektivitas penyembuhan luka sayat menggunakan salep ekstrak etanol daun senggani (Melastoma malabathricum L.) pada kelinci. J. Penelit. Farm. Herb. 2, 8–14 (2020).
Kaban, V. E. & Yusmarlisa, S. Uji aktivitas kandungan antioksidan pada daun bangun-bangun (Plectranthus amboinicus) secara spektrofotometri ultraviolet-visible. J. Farm. JFM 1, 16–20 (2018).
Hayatillah, R. & Hapsari, W. K. Anti-inflamasi tanaman kedondong (Spondias dulcis G. Forst.). J. Sains dan Pendidik. Biol. 2, 63–69 (2023).
Haqqi, A. N. & Wahyuni, W. Uji efektivitas ekstrak etanol kombinasi daun Afrika (Vernonia amygdalina Del.) dan daun kluwih (Artocarpus camansi) terhadap penurunan kadar asam urat pada mencit (Mus musculus). Pharm. Genius 2, 1–12 (2023).
Astika, R. Y. Uji aktivitas antiinflamasi ekstrak etanol daun kayu manis (Cinnamomum burmannii) pada mencit putih jantan. J. Ilm. Manuntung Sains Farm. dan Kesehat. 8, 14–23 (2022).
Nasri, N., Silalahi, J., Kaban, V. E. & Satria, D. A review on the benefits of probiotics as fermented food against several diseases. J. Funct. Food Nutraceutical 41–52 (2023).
Nasri, N. et al. Antibacterial potential of ethanolic extract of avocado leaves (Persea americana Mill.) against clinical isolate of Klebsiella pneumoniae and Proteus mirabilis. Trends Sci. 21, 7821 (2024).
Benn, C. Physiology and treatment of hyperuricemia and gout. In: Encyclopedia of Molecular Pharmacology 1234–1243 (Springer, 2022).
Almasarwah, S. Y., Oran, S. A. & Darwish, R. M. Efficacy of Nitraria retusa L. fruits aqueous and methanol extracts as antioxidant and anti-inflammatory activities on carrageenan-induced paw edema in rats. Trop. J. Nat. Prod. Res. 7 (2023).
Thao, T. T., Tu, P. T. & Men, T. T. A comparative study on polyphenol, flavonoid content, antioxidant and anti-inflammatory capacity of different solvent extract from Portulaca oleracea in carrageenan-induced paw edema in mice. Trop. J. Nat. Prod. Res. 7, 4152–4159 (2023).