Comparison of Examination Results of Acid-Fast Bacilli (AFB) Ziehl Neelsen (ZN) Method on BTA Preparations that are Directly Stained with Delayed Preparations
DOI:
https://doi.org/10.58549/nsmrj.v1i1.14Keywords:
Tuberculosis, Phlegm, BTAAbstract
Tuberculosis is a direct infectious disease caused by the bacterium Mycobacterium tuberculosis. Most tuberculosis germs attack the lungs, which is known as Pulmonary Tuberculosis (pulmonary TB), but can also affect other organs, which is called extrapulmonary TB. Pulmonary TB germs in the form of rods, have special properties, namely acid resistance to staining. The purpose of this study was to determine the results of examination of acid fast bacilli (AFB) by the Ziehl Neelsen method on smear preparations which were immediately stained and left to stand for three days. The research was conducted using a descriptive approach with a sample of 10 people using a purposive sampling technique. In this study, staining was carried out twice, namely direct staining and delayed staining for 3 days and examined under a microscope. The results showed that there was no difference between the preparedness that was immediately colored and the one that was postponed for three days.
References
Dewi, L. P. K. PEMERIKSAAN BASIL TAHAN ASAM UNTUK MEMBANTU MENEGAKKAN DIAGNOSIS PENYAKIT TUBERKULOSIS. Int. J. Appl. Chem. Res. 1, 16 (2020).
Nur Afiah, A. S. & Husen, A. H. PEMERIKSAAN SPUTUM BTA DENGAN HASIL PEMERIKSAAN GENEXPERT PADA PASIEN TB-MDR DI RSUD Dr. H. CHASAN BOESIRIE PERIODE TAHUN 2020. Kieraha Med. J. 2, (2021).
d’Arc Lyra Batista, J., de Fátima Pessoa Militão de Albuquerque, M., de Alencar Ximenes, R. A. & Rodrigues, L. C. Smoking increases the risk of relapse after successful tuberculosis treatment. Int. J. Epidemiol. 37, 841–851 (2008).
Barokah, A. A. N. Studi Deskriftif Pasien TB paru Berdasarkan Diagnosis Radiologi Di RSUP Wahidin Sudirohusodo Makassar September 2017-2018. (2020).
Muhammad Siri Dangnga, M. M. Pengaruh Merokok Terhadap Kejadian Konversi Sputum pada Penderita TB Paru Di kota Pare Pare. Jurnal Ilmiah Manusia Dan Kesehatan 3, 206–217 (2020).
Chen, P. et al. A Highly Efficient Ziehl-Neelsen Stain: Identifying De Novo Intracellular Mycobacterium tuberculosis and Improving Detection of Extracellular M. tuberculosis in Cerebrospinal Fluid. J. Clin. Microbiol. 50, 1166–1170 (2012).
Tansuphasiri, U., Boonrat, P. & Rienthong, S. Direct identification of Mycobacterium tuberculosis from sputum on Ziehl-Neelsen acid fast stained slides by use of silica-based filter combined with polymerase chain reaction assay. J. Med. Assoc. Thail. Chotmaihet Thangphaet 87, 180–189 (2004).
Husna, N. & Dewi, N. U. PERBANDINGAN HASIL PEMERIKSAAN MIKROSKOPIS BASIL TAHAN ASAM METODE DEKONTAMINASI DENGAN METODE TES CEPAT MOLEKULER. 12, 8.
Kalma, K. & Adrika, A. PERBANDINGAN HASIL PEMERIKSAAN BASIL TAHAN ASAM ANTARA SPESIMEN DAHAK LANGSUNG DIPERIKSA DENGA DITUNDA 24 JAM. J. Media Anal. Kesehat. 9, (2019).
Nelma, N. & Lumbantoruan, A. PENGARUH PENUNDAAN PENANGANAN SPUTUM TERHADAP HASIL PEMBACAAN SEDIAAN SECARA MIKROSKOPIS PADA PENDERITA TB DI UPT. LABORATORIUM KESEHATAN DAERAH PROVINSI SUMATERA UTARA. J. Ilm. PANNMED Pharm. Anal. Nurse Nutr. Midwivery Environ. Dent. 14, 95–100 (2019).
Ira Elvi Sulastri. Uji Sensitivitas dan Spesifisitas Pewarnaan Ziehl-Neelsen Pada Penderita Suspek Tubeculosis Paru Di Puskesmas Pancur Batu Deli Serdang. (2019).
World Health Organization. WHO guidelines on tuberculosis infection prevention and control: 2019 update. (World Health Organization, 2019).